Antibacterial Effects of Lactic Acid Bacteria Cell Free Extract on the Growth of Local Isolates of Listeria moncytogenes from some Food Sources

Zainab Alzubaidy, Shean Kakey, Jassim Ali
J. Sci   unpublished
The present study aimed to determine the antibacterial activity of lactic acid bacteria metabolites against Listeria monocytogenes which was isolated from some foods in local markets of Erbil and Koya cities; 225 food samples were examined for the presence of Listeria species particularly L. monocytogenes from October 2010 to March 2011. The studied samples included 70 samples of raw chicken meat, 55 samples of raw milk, 50 samples of cheese and 50 samples of raw red meat. According to motility
more » ... test, hemolysin production, sugar fermentation test, 8 isolates of L. monocytogenes were identified, 1 (2 %) isolate from cheese, 2 (4 %) isolates from red meat and 5 (7.3 %) isolates from chicken meat. The percentages of contaminated foods for Listeria species from chicken meat, raw milk, cheese and red meat were 20, 9, 20 and 22% respectively. With respect to the biocontrolling attempt, the effect of cell free extract (CFE) of lactic acid bacteria (LBA) which is considered as safe against isolated L. monocytogene was used as biocontrolling agent. The CFE of lactic acid bacteria which isolated from local dairy products, was tested for its controlling ability by agar diffusion method, the CFE showed inhibition zone from (15-23 mm) in diameter. Also the minimum inhibitory concentration MIC of CFE was determined at three additive percentages (0.5, 1.0 and 1.5ml to 10 ml of nutrient broth), the inhibition percentage ranged from (28.75-48.97) at (0.5 %), (52.13-95.55) at (1 %) and (82.93-98.15) at (1.5 %) additive percentage of CFE of LAB. ‫ــــــــــــــــــــــــــــــــــــــــــــــــــــ‬ Zainab M. Alzubaidy et al. 20 ‫ﺒﻜﺘﺭﻴﺎ‬ ‫ﻨﻤﻭ‬ ‫ﻋﻠﻰ‬ ‫ﺍﻟﻠﺒﻨﻴﻙ‬ ‫ﺤﺎﻤﺽ‬ ‫ﻟﺒﻜﺘﺭﻴﺎ‬ ‫ﺍﻻﻴﻀﻴﺔ‬ ‫ﻟﻠﻨﻭﺍﺘﺞ‬ ‫ﺍﻟﺘﺜﺒﻴﻁﻲ‬ ‫ﺍﻟﺘﺄﺜﻴﺭ‬ Listeria monocytogenes ‫ﻤﺼﺎﺩﺭ‬ ‫ﺒﻌﺽ‬ ‫ﻤﻥ‬ ‫ﺍﻟﻤﻌﺯﻭﻟﺔ‬ ‫ﺍﻷﻏﺫﻴﺔ‬ ‫ﺍﻟﻤﻠﺨﺹ‬ ‫ﻫﺩﻓ‬ ‫ﺍﻟﺤﺎﻟﻴﺔ‬ ‫ﺍﻟﺩﺭﺍﺴﺔ‬ ‫ﺕ‬ ‫ﺇﻟﻰ‬ ‫ﺍﻟﺘﺜﺒ‬ ‫ﺍﻟﻔﻌﺎﻟﻴﺔ‬ ‫ﺘﺤﺩﻴﺩ‬ ‫ﺒﻜﺘﺭﻴﺎ‬ ‫ﻀﺩ‬ ‫ﺍﻻﻜﺘﻴﻙ‬ ‫ﺤﺎﻤﺽ‬ ‫ﻟﺒﻜﺘﺭﻴﺎ‬ ‫ﺍﻻﻴﻀﻴﺔ‬ ‫ﻟﻠﻨﻭﺍﺘﺞ‬ ‫ﻴﻁﻴﺔ‬ Listeria monocytogen ‫ﻭﻜﻭﻴﻪ‬ ‫ﺍﺭﺒﻴل‬ ‫ﻤﺩﻴﻨﺔ‬ ‫ﺍﺴﻭﺍﻕ‬ ‫ﻓﻲ‬ ‫ﺍﻟﻤﺘﻭﺍﺠﺩﺓ‬ ‫ﺍﻟﻤﺤﻠﻴﺔ‬ ‫ﺍﻻﻏﺫﻴﺔ‬ ‫ﺒﻌﺽ‬ ‫ﻤﻥ‬ ‫ﺍﻟﻤﻌﺯﻭﻟﺔ‬ ، ‫ﺠﻤﻌﺕ‬ 225 ‫ﺍﻟﻤﺤﻠﻴﺔ‬ ‫ﺍﻻﺴﻭﺍﻕ‬ ‫ﻤﻥ‬ ‫ﻋﺸﻭﺍﺌﻲ‬ ‫ﺒﺸﻜل‬ ‫ﻤﺨﺘﻠﻔﺔ‬ ‫ﻏﺫﺍﺌﻴﻪ‬ ‫ﻨﻤﺎﺫﺝ‬ ‫ﻤﻥ‬ ‫ﻋﻴﻨﺔ‬. ‫ﹸ‬ ‫ﺍﻟﻤﺩﺭﻭﺴﺔ‬ ‫ﹸ‬ ‫ﺍﻟﻌﻴﻨﺎﺕ‬ ‫ﹾ‬ ‫ﻨﺕ‬ ‫ﹶﻀﻤ‬ ‫ﺘ‬ 70 ‫ﻤﻥ‬ ِ ‫ﻨﺔ‬ ‫ﻋﻴ‬ ،ِ ‫ﺍﻟﺨﺎﻡ‬ ِ ‫ﺍﻟﺩﺠﺎﺝ‬ ِ ‫ﻟﺤﻡ‬ 55 ،ِ ‫ﺍﻟﺨﺎﻡ‬ ِ ‫ﺍﻟﺤﻠﻴﺏ‬ ‫ﻤﻥ‬ ِ ‫ﻨﺔ‬ ‫ﻋﻴ‬ 50 ‫ﻭ‬ ِ ‫ﺍﻟﺠﺒﻥ‬ ‫ﻤﻥ‬ ِ ‫ﻨﺔ‬ ‫ﻋﻴ‬ 50 ِ ‫ﺍﻟﻠﺤﻭﻡ‬ ‫ﻤﻥ‬ ِ ‫ﻨﺔ‬ ‫ﻋﻴ‬ ِ ‫ﺍﻟﺨﺎﻡ‬ ِ ‫ﺍﻟﺤﻤﺭﺍﺀ‬. ‫ﺍﻟﻠﺴﺘﻴﺭﻴﺎ‬ ‫ﺒﺠﺭﺜﻭﻤﺔ‬ ‫ﺘﻠﻭﺜﻬﺎ‬ ‫ﻟﻤﺩﻯ‬ ‫ﺍﻟﺤﺎﻟﻴﺔ‬ ‫ﺍﻟﺩﺭﺍﺴﺔ‬ ‫ﻓﻲ‬ ‫ﺍﻟﺨﺎﻀﻌﺔ‬ ‫ﺍﻟﻌﻴﻨﺎﺕ‬ ‫ِﺠﻤﻴﻊ‬ ‫ﹾ‬ ‫ِﺼﺕ‬ ‫ﹸﺤ‬ ‫ﻓ‬ ‫ﻭﺨﺼﻭﺼﺎ‬) Listeria monocytogens .(‫ﺠﺭﺜﻭﻤﺔ‬ ‫ﺤﺭﻜﺔ‬ ‫ﻹﺨﺘﺒﺎﺭﺍﺕ‬ ‫ﹰ‬ ‫ﻁﺒﻘﺎ‬ L. monocytogens ‫ﺍﻨﺘﺎﺠﻬﺎ‬ ‫ﻭ‬ ‫ﺘﺸﺨﻴﺹ‬ ‫ﺘﻡ‬ ‫ﺍﻟﺴﻜﺭﻴﺎﺕ‬ ‫ﺘﺨﻤﻴﺭ‬ ‫ﻋﻠﻰ‬ ‫ﻭﻗﺩﺭﺘﻬﺎ‬ ‫ﻟﻠﺩﻡ‬ ‫ﺍﻟﺤﺎل‬ ‫ﻻﻨﺯﻴﻡ‬ 8 ‫ﻭﺍﺤﺩﻩ‬ ‫ﻋﺯﻟﺔ‬ ‫ﺒﻤﻌﺩل‬ ،‫ﻟﻠﺠﺭﺜﻭﻤﺔ‬ ‫ﻋﺯﻻﺕ‬ ‫ﻭﺒﻨﺴﺒﺔ‬ 2 % ‫ﻭﺒﻨﺴﺒﺔ‬ ‫ﻭﻋﺯﻟﺘﻴﻥ‬ ،‫ﺍﻟﺠﺒﻥ‬ ‫ﻋﻴﻨﺎﺕ‬ ‫ﻤﻥ‬ 4 % ‫ﺒﻨﺴﺒﺔ‬ ‫ﻋﺯﻻﺕ‬ ‫ﻭﺨﻤﺴﺔ‬ ،ِ ‫ﺍﻷﺤﻤﺭ‬ ِ ‫ﺍﻟﻠﺤﻡ‬  ‫ِﻥ‬ ‫ﻤ‬ ‫ﻋﻴﻨﺎﺕ‬ ‫ﻤﻥ‬ 7.3 % ‫ﺍﻟﺩﺠﺎﺝ‬ ‫ﻟﺤﻡ‬ ‫ﻤﻥ‬. ‫ﺍﻟ‬ ِ ‫ﻟﺤﻡ‬ ‫ﻟﻌﻴﻨﺎﺕ‬ ‫ﺍﻟﺘﻠﻭﺙ‬ ‫ﻨﺴﺏ‬ ‫ﺘﺭﺍﻭﺤﺕ‬ ‫ﺠﺒ‬ ،‫ﺨﺎﻡ‬ ‫ﺤﻠﻴﺏ‬ ،ِ ‫ﺩﺠﺎﺝ‬ ‫ـ‬ ‫ﺒﺠﺭﺜﻭﻤﺔ‬ ‫ﺃﺤﻤﺭ‬ ‫ﻭﻟﺤﻡ‬ ‫ﻥ‬ Listeria 20 ، 9 ، 20 ‫ﻭ‬ 22 % ‫ﺍﻟﺤﻴﻭﻴﺔ‬ ‫ﺍﻟﺴﻴﻁﺭﺓ‬ ‫ﺩﺭﺍﺴﺔ‬ ‫ﺒﺨﺼﻭﺹ‬ ‫ﺍﻤﺎ‬ ‫ﺍﻟﺘﻭﺍﻟﻲ‬ ‫ﻋﻠﻰ‬ biocontrolling ‫ﻭﺒﻘﺎﺀ‬ ‫ﻨﻤﻭ‬ ‫ﻋﻠﻰ‬) Listeria monocytogenes (‫ﺍﻟﺒﻜﺘﻴﺭﻴﺔ‬ ‫ﺍﻟﻤﺯﺭﻋﺔ‬ ‫ﺭﻭﺍﺸﺢ‬ ‫ﺍﺴﺘﺨﺩﻡ‬ ‫ﺍﺫ‬ ، CFE ‫ﺤﺎﻤﺽ‬ ‫ﻟﺒﻜﺘﻴﺭﻴﺎ‬ ‫ﺍﻟﻠﺒﻨﻴﻙ‬ LAB ‫ﻭﺍﻟ‬ ‫ﺘ‬ ‫ﻭﻁﺒﻴﻌﻴ‬ ‫ﺁﻤﻨﺔ‬ ‫ﺘﻌﺘﺒﺭ‬ ‫ﻲ‬ ‫ﻋﺯﻻﺕ‬  ‫ﻀﺩ‬ ،‫ﺔ‬) Listeria monocytogenes (‫ﺍﻟﺤﻴﻭﻴﺔ‬ ‫ﺍﻟﺴﻴﻁﺭﺓ‬ ‫ﻓﻲ‬. ‫ﺍﻟﻤﺤﻠﻴﺔ‬ ‫ﺍﻻﻟﺒﺎﻥ‬ ‫ﻤﻨﺘﺠﺎﺕ‬ ‫ﺒﻌﺽ‬ ‫ﻤﻥ‬ ‫ﺍﻟﻤﻌﺯﻭﻟﺔ‬ ‫ﺍﻟﻠﺒﻨﻴﻙ‬ ‫ﺤﺎﻤﺽ‬ ‫ﺒﻜﺘﻴﺭﻴﺎ‬ ‫ﻤﻥ‬ ‫ﺍﻟﺭﺍﺸﺢ‬ ‫ﺍﺴﺘﺨﻠﺹ‬ ‫ﺍﺫ‬. ‫ﻗﺩﺭﺓ‬ ‫ﺍﺨﺘﺒﺎﺭ‬ ‫ﺘﻡ‬ ‫ﻟ‬ ‫ﺍﻟﺤﻴﻭﻱ‬ ‫ﺍﻟﺘﺜﺒﻴﻁ‬ ‫ـ‬ LAB ‫ﻀﻌﻔﻴﻥ‬ ‫ﺍﻟﻰ‬ ‫ﺍﻟﺭﺍﺸﺢ‬ ‫ﺘﺭﻜﻴﺯ‬ ‫ﻓﻴﻬﺎ‬ ‫ﺘﻡ‬ ‫ﻭﺍﻟﺘﻲ‬ ‫ﺒﺎﻟﺤﻔﺭ‬ ‫ﺍﻻﻨﺘﺸﺎﺭ‬ ‫ﺒﻁﺭﻴﻘﺔ‬. ‫ﺍﺫ‬ ‫ﺃﻅﻬﺭ‬ ‫ﺕ‬ ‫ﺍﻻ‬ ‫ﻨﺘﻴﺠﺔ‬ ‫ﻤﻥ‬ ‫ﺍﻟﻤﺴﺘﺨﻠﺹ‬ ‫ﺍﻟﺭﺍﺸﺢ‬ ‫ﺍﻥ‬ ‫ﻫﺫﺍ‬ ‫ﺨﺘﺒﺎﺭ‬ LAB ‫ﻤﻨﻁﻘﺔ‬ ‫ﻗﻁﺭ‬ ‫ﺘﺭﺍﻭﺡ‬ ‫ﺍﺫ‬ ،‫ﺍﻟﺒﻜﺘﻴﺭﻱ‬ ‫ﺍﻟﻨﻤﺅ‬ ‫ﺘﺜﺒﻴﻁ‬ ‫ﻋﻠﻰ‬ ‫ﺍﻟﻘﺩﺭﺓ‬ ‫ﺍﻟﺘﺜﺒﻴﻁ‬) 15-23 ‫ﻤﻠﻴﻤﺘﺭ‬ .(‫ﺍﻟﻤﺜﺒﻁ‬ ‫ﺍﻻﺩﻨﻰ‬ ‫ﺍﻟﺘﺭﻜﻴﺯ‬ ‫ﺤﺩﺩ‬ CFE ‫ﺍﻻﻀﺎﻓﺎﺕ‬ ‫ﻤﻥ‬ ‫ﻨﺴﺏ‬ ‫ﺜﻼﺙ‬ ‫ﺒﺎﺴﺘﺨﺩﺍﻡ‬) 0.5 , 1.0 ‫ﻭ‬ 1.5 (% ‫ﻟ‬ ‫ـ‬ CFE ‫ﺘﺜﺒﻴﻁ‬ ‫ﻨﺴﺒﺔ‬ ‫ﺍﻟﺭﺍﺸﺢ‬ ‫ﺍﻅﻬﺭ‬ ‫ﺍﺫ‬ ،) 28.75-48.97 (‫ﺍﻻﻀﺎﻓﺔ‬ ‫ﻨﺴﺒﺔ‬ ‫ﻋﻨﺩ‬) 0.5 (% ،) 95.55-52.13 (‫ﺍﻻﻀﺎﻓﺔ‬ ‫ﻨﺴﺒﺔ‬ ‫ﻋﻨﺩ‬) 1 (% ‫ﻭ‬) 98.15-82.93 (‫ﺍﻻﻀﺎﻓﺔ‬ ‫ﻨﺴﺒﺔ‬ ‫ﻋﻨﺩ‬) 1.5 .(% ‫ﺍﻟﺩﺍﻟﺔ‬ ‫ﺍﻟﻜﻠﻤﺎﺕ‬ : Listeria moncytogenes ، ‫ﺍﻟﻼﻜﺘﻴﻙ‬ ‫ﺤﺎﻤﺽ‬ ‫ﺒﻜﺘﺭﻴﺎ‬ .
fatcat:n6lw7eousbg37eingls2u4oqg4