Fate and location of Vibrio anguillarum in tissues of artificially infected Ayu (Plecoglossus altivelis)

Kiyokuni MUROGA, Margarita C. DE LA CRUZ
1987 Gyobyo kenkyu - Fish pathology  
Ayu (Plecoglossus altivelis) were infected with Vibrio anguillarum by a water-born method . At 6, 12, 18, 36,[38][39][40][41][42][43][44][45] and 48 h (dead) after infection , fish were sampled to determine the fate and location of the bacterium in various tissues by viable cell count and the enzyme-labelled antibody technique (ELAT). V. anguillarum was first detected in the skin at 12 h by bacterial isolation . It appeared in the muscle, spleen and liver at 24 h, but was not isolated from the
more » ... isolated from the gills or intestine until 36 h or 38-45 h. The same trend in the fate of the pathogen was confirmed by ELAT , and the cells were found in dermal layer of the skin from the early stage (12 h) of infection . Based on these observations, it was concluded that the first colonization site of V. anguillarum in ayu was the skin.
doi:10.3147/jsfp.22.99 fatcat:bzrviqbuy5abplegfi2apuhuum