Isolation of hyper-protease-producing mutants in Bacillus subtilis natto
Bacillus subtilis nattoに於けるプロテアーゼ高生産変異株の分離

Noriyuki YAMAGUCHI, Yuichi NAKAMURA, Takenori OKUHIRA, Masaaki KITANO, Hideki MORISHITA
1991 Seikatsu Eisei (Journal of Urban Living and Health Association)  
Hyper-protease producing mutants were isolated from Bacillus subtilis MAFF10-08100 a natto Miura strain. All seven mutants showed low growth while protease activity was high with soybean protein as the substrate compared to the parent strain. Protease production depended on growth and was maximum in the stationary phase. The extracellular protease activity of mutant No .32 showed a maximum that was 3.6-fold times that strain cells.
doi:10.11468/seikatsueisei1957.35.83 fatcat:bmjnyyjpfbaspixri7xr3jrla4