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A new calcium-responsive tag based on a consensus sequence found in the natural repeat-in-toxin (RTX) domain is presented. This calcium-responsive tag works under gentler reaction conditions than existing approaches and can be removed through protease cleavage, resulting in a pure, active target protein. Reports www.doi:10.2144/000114010 pmid:23581466 fatcat:n7erzgojdngrtnn4sc3jynq6km