Competencia morfogénica de embriones maduros de Pinus radiata cultivados in vitro y su relación con la posición del cono en el árbol

Manuel Sánchez-Olate, Jaime Zapata Valenzuela, Patricia Sáez Delgado, Darcy Ríos Leal, Scarlette Spiercolli Henríquez, Carolina Alvarez Maldini, Catherine Delaveau, Guillermo Pereira Cancino
2008 Bosque (Valdivia)  
Competencia morfogénica en embriones de P. radiata BOSQUE 29(3): 212-216, 2008 Competencia morfogénica de embriones maduros de Pinus radiata cultivados in vitro y su relación con la posición del cono en el árbol Morphogenic competence of mature in vitro cultured Pinus radiata embryos and their relation with cone position in the tree SUMMARY The micropropagation of zygotic material collected from adult trees constitutes an important alternative in massive propagation of select phenotypes,
more » ... phenotypes, appropriate for cloning programs implemented by forest companies. Using this technique, embryonic axes extracted from lignified cones located at different heights in the trees (basal, medial and apical zones) where in vitro introduced, with the objective of analyzing the possible relation between the physiological state of the forming tissue and the response obtained in the micropropagation of mature Pinus radiata embryos. Embryos obtained from cones located in the basal zone of the donor tree, demonstrated the best results in regards to germination (95%), survival (100%), microshoot proliferation (1.7 buds/microstems) and microshoot elongation (25 mm), which allowed to establish a relation between the development states of the explants and their response in artificial in vitro conditions, supplying background information for the implementation of a classification criteria of the collected material to be used in tissue cultures, due to the variable responses obtained according to the origin of the embryos from donor trees. RESUMEN La micropropagación de material embrionario colectado de árboles adultos constituye una alternativa importante en la propagación masiva de fenotipos selectos, adecuados para los programas de clonación implementados por las empresas forestales. Utilizando esta técnica, ejes embrionarios maduros extraídos desde conos lignificados ubicados a diferentes alturas en el árbol (zonas basal, media Palabras clave: Pinus radiata, embriones zigóticos, revigorización, edad fisiológica.
doi:10.4067/s0717-92002008000300004 fatcat:5t5ldfnjgfhnfaxc5b532qnqj4