Local cholera immunity in mice: intestinal antitoxin-containing cells and their correlation with protective immunity

S Lange, H A Hansson, S O Molin, H Nygren
1979 Infection and Immunity  
numbers after two oral immunizations and increased sixto eightfold with two additional oral boosters, whereas further oral immunizations caused no additional stimulation. Intravenous immunizations had to be repeated seven times before ACC could be detected. After two oral immunizations, most of the ACC were found in the proximal part of the small intestine and no ACC were seen in the colon. This uneven distribution of ACC within the small intestine was eliminated after four oral immunizations,
more » ... ral immunizations, when ACC could also be detected in the colon. The ACC response after four oral immunizations demonstrated a peak at 4 days with far fewer cells present at 2 and 7 days. Electron microscopic studies showed that the ACC were mature plasma cells with the staining localized to the endoplasmic reticulum. Regression analysis of the relationship between the number of ACC and the magnitude of protective immunity against intestinal challenge with cholera toxin indicates a highly significant correlation (r = 0.91, P < 0.001). 743 on May 7, 2020 by guest http://iai.asm.org/ Downloaded from
doi:10.1128/iai.23.3.743-750.1979 fatcat:7xjq5dkfw5gafbmjrjrae4vunu