Glucose-induced glutathione reduction in mitochondria is involved in the first phase of pancreatic β-cell insulin secretion

Xiaojing Liu, Shuai Han, Ying Yang, Jiuhong Kang, Jiarui Wu
2015 Biochemical and Biophysical Research Communications - BBRC  
Glucose can acutely reduce mitochondrial glutathione redox state in rat islets. However, whether glucose-stimulated mitochondrial glutathione redox state relates to glucose-stimulated insulin secretion (GSIS) remains unknown. We used genetically encoded redox-sensitive GFPs to target the mitochondria to monitor glutathione redox changes during GSIS in rat pancreatic b-cells. The results showed that mitochondrial glutathione was more reduced during GSIS, whereas inhibition of this glutathione
more » ... uction impaired insulin secretion. In isolated rat pancreatic islets glutathione reduction in mitochondria and the first phase of GSIS were concurrence at the early stage of glucose-stimulation. Our results suggest that the glucose-induced glutathione reduction in mitochondria is primarily required for the first phase of GSIS.
doi:10.1016/j.bbrc.2015.07.016 pmid:26164230 fatcat:25fye4qs2fhznbwdefd6olzd4q