SIMULTANEOUS ESTIMATION OF LINAGLIPTIN AND METFORMIN HCL IN HUMAN PLASMA BY RP-HPLC METHOD

Balamurugan K, Kirtimaya Mishra, Suresh R
2019 International Research Journal of Pharmacy  
A new and simple method was developed for quantitative determination of Linagliptin (LINA) and Metformin (MET) spiked with plasma using an Onyx C18 Monolithic column connected with an Onyx C18 guard cartridge and PDA detection at 220 nm. The optimized mobile phase acetonitrile (ACN), methanol (MeOH), formic acid (HCOOH) was pumped at 0.892 mL/min. The method was linear between 0.02-0.1 µg/mL, statistically validated for its linearity, precision, and accuracy. The precision was found to be less
more » ... han 1% in the assay method. It was found that the additives in the commercial tablet did not interfere with the method. The currently developed method can routinely use for the estimation of LINA and MET related compounds from the tablet dosage form spiked with plasma.
doi:10.7897/2230-8407.100128 fatcat:d7ibusqnmzairlk2jnfptngjeq