Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria

Jasmina Vidic, Priya Vizzini, Marisa Manzano, Devon Kavanaugh, Nalini Ramarao, Milica Zivkovic, Vasa Radonic, Nikola Knezevic, Ioanna Giouroudi, Ivana Gadjanski
2019 Sensors  
Foodborne pathogenic bacteria present a crucial food safety issue. Conventional diagnostic methods are time-consuming and can be only performed on previously produced food. The advancing field of point-of-need diagnostic devices integrating molecular methods, biosensors, microfluidics, and nanomaterials offers new avenues for swift, low-cost detection of pathogens with high sensitivity and specificity. These analyses and screening of food items can be performed during all phases of production.
more » ... his review presents major developments achieved in recent years in point-of-need diagnostics in land-based sector and sheds light on current challenges in achieving wider acceptance of portable devices in the food industry. Particular emphasis is placed on methods for testing nucleic acids, protocols for portable nucleic acid extraction and amplification, as well as on the means for low-cost detection and read-out signal amplification.
doi:10.3390/s19051100 fatcat:326brigc3zejdenu4jeewfsywq