Sub-Chronic Effects of s-Limonene on Brain Neurotransmitter Levels and Behavior of Rats

Wenjun ZHOU, Miyuki YOSHIOKA, Hidehiko YOKOGOSHI
2009 Journal of Nutritional Science and Vitaminology  
The present study was designed to gain insight into the effects of s -limonene on the brain after 1-wk administration. For this purpose, neurotransmitters such as dopamine (DA), serotonin (5-HT), ␥ -aminobutyric acid (GABA), glutamic acid (Glu) and some of their metabolites (DOPAC and 5-HIAA) were determined by HPLC-ECD and amino acid analyzer after 1-wk administration of s -limonene of different concentrations (0, 5, 25, 50 mg/ kg). Significant changes, such as GABA, 5-hydroxyindoleacetic acid
more » ... (5-HIAA) and 5-HT, were confirmed. At the same time, basal hypothalamic-pituitary-adrenal (HPA) activity after 1-wk administration of s -limonene was evaluated by corticosterone. Considering the increment of GABA and the changes of other neurotransmitters, anti-stress effects after 1wk administration were observed. The experimental results showed that s -limonene could inhibit HPA activity under physical stress and this anti-stress effect of s -limonene may act through the GABA A receptor.
doi:10.3177/jnsv.55.367 fatcat:snkanmoaifab7hifqxmompbzp4