Integrin LFA-1 regulates cell adhesion via transient clutch formation

Munenori Ishibashi, Yukihiro Miyanaga, Satomi Matsuoka, Jun Kozuka, Yuichi Togashi, Tatsuo Kinashi, Masahiro Ueda
2015 Biochemical and Biophysical Research Communications - BBRC  
Integrin LFA-1 regulates immune cell adhesion and trafficking by binding to ICAM-1 upon chemokine stimulation. Integrin-mediated clutch formation between extracellular ICAM-1 and the intracellular actin cytoskeleton is important for cell adhesion. We applied single-molecule tracking analysis to LFA-1 and ICAM-1 in living cells to examine the ligand-binding kinetics and mobility of the molecular clutch under chemokine-induced physiological adhesion and Mn 2þ -induced tight adhesion. Our results
more » ... how a transient LFA-1-mediated clutch formation that lasts a few seconds and leads to a transient lowermobility is sufficient to promote cell adhesion. Stable clutch formation was observed for Mn 2þinduced high affinity LFA-1, but was not required for physiological adhesion. We propose that fast cycling of the clutch formation by intermediate-affinity integrin enables dynamic cell adhesion and migration.
doi:10.1016/j.bbrc.2015.06.155 pmid:26143530 fatcat:rjw3htukofebpcnrtacxf4wbki