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Computational codon optimization of synthetic gene for protein expression
2012
BMC Systems Biology
The construction of customized nucleic acid sequences allows us to have greater flexibility in gene design for recombinant protein expression. Among the various parameters considered for such DNA sequence design, individual codon usage (ICU) has been implicated as one of the most crucial factors affecting mRNA translational efficiency. However, previous works have also reported the significant influence of codon pair usage, also known as codon context (CC), on the level of protein expression.
doi:10.1186/1752-0509-6-134
pmid:23083100
pmcid:PMC3495653
fatcat:icp2ykmycreyhdg4t7skoiwy44