Detection of Tricyclic Antidepressants in Whole Blood by Headspace Solid-Phase Microextraction and Capillary Gas Chromatography

X.-P. Lee, T. Kumazawa, K. Sato, O. Suzuki
1997 Journal of Chromatographic Science  
A simple method for the extraction of four tricyclic antidepressants from whole blood by headspace solid-phase microextraction (SPME) is presented. The whole blood samples contain four drugs (amitriptyline, chlorimipramine, imipramine, and trimipramine) and are heated at 100°C in a septum-capped vial in the presence of distilled water and NaOH solution; a polydimethylsiloxane-coated SPME fiber is exposed to the headspace of the vial to allow adsorption of the drugs before capillary gas
more » ... raphy (GC) with flame-ionization detection. The headspace SPME-GC produces intense peaks for each drug with very little background noise. Recoveries of the four drugs by the present method are 5.3-12.9%. The calibration curves for the drugs show linearity in the range of 31-1000 ng/0.5 mL The detection limits of each drug are 16-25 ng/0.5 ml. Imipramine is detectable from rat blood 5 h after oral administration of imipramine (500 mgAg body weight); the concentration is 1.44 ± 0.209 μg/mL.
doi:10.1093/chromsci/35.7.302 pmid:9218344 fatcat:opv6ynfp3jdq3awb5xechgdh5q