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C9orf72 and RAB7L1 regulate vesicle trafficking in amyotrophic lateral sclerosis and frontotemporal dementia

Yoshitsugu Aoki, Raquel Manzano, Yi Lee, Ruxandra Dafinca, Misako Aoki, Andrew G. L. Douglas, Miguel A. Varela, Chaitra Sathyaprakash, Jakub Scaber, Paola Barbagallo, Pieter Vader, Imre Mäger (+10 others)
2017 Brain  

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https://web.archive.org/web/20180728013814/https://watermark.silverchair.com/awx024.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAaUwggGhBgkqhkiG9w0BBwagggGSMIIBjgIBADCCAYcGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMLIIe1o4QhOrvW6V1AgEQgIIBWFwKcaS--z3ZsbAMgRy6L3mV1-f6f4a6mpGH3OQ6q9CQ4_yHk1Kc7J39uOiGqCNlF_NoPuPhvrrr8Om9pku7959KTttEt9cjDuV3hlD1WSfPhHJ1INA_0rT-vK8_L-apPAGy5sPwHdSUpYPMPrWaJGUw6DadEMRWERT8AEbncpB0bF-uUMwfHcbPGZhyD-XvvQmyaQ59vkTwz2PrKbYHHIiMjwFDHg8IoHCGWtYn56PodWC5212dCCaryEPosK3yqS0WR2yEe_wSfiEgWzFKU8VUq3yt0LgBfZ18lt1-Bgx_YrofFzd3p8g41MqCm031FDzCZzFk81sYuWg_LeK_-fvcFlIfvCc1dVn2JWcGGpjcca1vntfVkJoJJJRhmjUVFQWlmxyKWpbXpcG-XzYkgSxBmuCPVdXYsDvengDzk1A9dYnFaCvvqNaxQ8iA2D5wIQObLC7pqcNR

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à These authors contributed equally to this work. A non-coding hexanucleotide repeat expansion in intron 1 of the C9orf72 gene is the most common cause of amyotrophic lateral sclerosis and frontotemporal dementia (C9ALS/FTD), however, the precise molecular mechanism by which the C9orf72 hexanucleotide repeat expansion directs C9ALS/FTD pathogenesis remains unclear. Here, we report a novel disease mechanism arising due to the interaction of C9ORF72 with the RAB7L1 GTPase to regulate vesicle
more » ... icking. Endogenous interaction between C9ORF72 and RAB7L1 was confirmed in human SH-SY5Y neuroblastoma cells. The C9orf72 hexanucleotide repeat expansion led to haploinsufficiency resulting in severely defective intracellular and extracellular vesicle trafficking and a dysfunctional trans-Golgi network phenotype in patient-derived fibroblasts and induced pluripotent stem cell-derived motor neurons. Genetic ablation of RAB7L1or C9orf72 in SH-SY5Y cells recapitulated the findings in C9ALS/FTD fibroblasts and induced pluripotent stem cell neurons. When C9ORF72 was overexpressed or antisense oligonucleotides were targeted to the C9orf72 hexanucleotide repeat expansion to upregulate normal variant 1 transcript levels, the defective vesicle trafficking and dysfunctional trans-Golgi network phenotypes were reversed, suggesting that both loss-and gain-of-function mechanisms play a role in disease pathogenesis. In conclusion, we have identified a novel mechanism for C9ALS/FTD pathogenesis highlighting the molecular regulation of intracellular and extracellular vesicle trafficking as an important pathway in C9ALS/FTD pathogenesis. Abbreviations: ALS = amyotrophic lateral sclerosis; FTD = frontotemporal dementia; HRE = hexanucleotide repeat expansion; iPSC = induced pluripotent stem cell; NTA = nanoparticle tracking analysis
doi:10.1093/brain/awx024 pmid:28334866 fatcat:b2c3ddgesfgfplojdd2utwokl4
Citation

Yoshitsugu Aoki, Raquel Manzano, Yi Lee, Ruxandra Dafinca, Misako Aoki, Andrew G. L. Douglas, Miguel A. Varela, Chaitra Sathyaprakash, Jakub Scaber, Paola Barbagallo, Pieter Vader, Imre Mäger, Kariem Ezzat, Martin R. Turner, Naoki Ito, Samanta Gasco, Norihiko Ohbayashi, Samir El Andaloussi, Shin'ichi Takeda, Mitsunori Fukuda, Kevin Talbot, Matthew J. A. Wood. "C9orf72 and RAB7L1 regulate vesicle trafficking in amyotrophic lateral sclerosis and frontotemporal dementia." Brain 140.4 (2017) 887-897