Further evidence that CP-AMPARs are critically involved in synaptic tag and capture at hippocampal CA1 synapses [post]

Pojeong Park, Heather Kang, John Georgiou, Min Zhuo, Bong-Kiun Kaang, Graham L Collingridge
2020 unpublished
The synaptic tag and capture (STC) hypothesis provides an important theoretical basis for understanding the synaptic basis of associative learning. We recently provided pharmacological evidence that calcium-permeable AMPA receptors (CP-AMPARs) are a crucial component of this process. Here we have investigated two predictions that arise on the basis of CP-AMPARs serving as a trigger of the STC effect. Firstly, we compared the effects of the order in which we delivered a strong theta burst
more » ... theta burst stimulation (TBS) protocol (75 pulses) and a weak TBS protocol (15 pulses) to two independent inputs. We only observed a significant STC effect when the strong preceded the weak TBS. Second, we found that pausing stimulation following either the sTBS or the wTBS for ~20 min largely eliminates the STC effect. These observations are exactly as predicted for a process that is triggered by the synaptic insertion of CP-AMPARs and provide a framework for establishing the underlying molecular mechanism.
doi:10.21203/rs.3.rs-61570/v1 fatcat:3sqa6hgoabdt3dtxn7kzeu7hei