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UC Riverside International Organization of Citrus Virologists Conference Proceedings (1957-2010) Title Huanglongbing (Greening) in Vietnam: Detection of Liberobacter asiaticum by DNA-Hybridization with Probe in 2.6 and PCR-Amplification of 16S Ribosomal DNA Publication Date
1957
Journal International Organization of Citrus Virologists Conference Proceedings
unpublished
Leaf samples were collected from 55 citrus trees from north, central and south Vietnam in January-February 1995, and analyzed by PCR amplification, DNA hybridization and immunofluorescence for the presence of the Huanglongbing (HLB) (greening) liberobacter. Fortyeight of the 55 samples hybridized with probe In 2.6 which is specific for Liberobacter asiaticum. No hybridization was obtained with probe As 1.7, specific for L. africanum. Forty-five of 47 samples analyzed by PCR gave the 1,160 bp
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