Observation of Biochemical Imaging Changes in Human Pancreatic Cancer Tissue using Fourier-transform Infrared Microspectroscopy

Ying-Jen Chen, Yih-Dih Cheng, Hsin-Yi Liu, Paul-Yann Lin, Chia-Siu Wang
Fourier-transform infrared (FT-IR) microspectroscopic mapping can be used to distinguish between different tissue structures, and to increase the image contrast between normal and cancerous regions of a given tissue sample. This study demonstrates the biochemical changes associated with a consistent link between cancerous tissue and various molecular changes in the IR spectra of human pancreatic cancer tissue using FT-IR mapping. Tissue samples were obtained immediately after resection in a
more » ... resection in a patient who underwent a distal pan-createctomy including the pancreatic body and tail. The biochemical imaging changes of lipids, proteins, and nucleic acids in human pancreatic cancer tissue were analyzed via FT-IR microspectroscopy, using imaging, mapping, and line scan techniques. The intensities and frequencies of the absorption bands in the IR spectra of human pancreatic cancerous tissue were markedly reduced and shifted, particularly in the amide bands of protein and CH2 and CH3 stretching vibrations of lipids. The cancerous tissue contained significant protein content, and the distributions of DNA and lipid were very low, indicating low amounts of nucleic acids and lipids in human pancreatic cancer tissue. The analytical results indicate that these FT-IR microspectroscopic biochemical images reflect the distribution of cell components , which could be correlated with stained tissue in adenocarcinoma in pancreatic tissues. This study with samples of noncancerous and cancerous pancreatic tissues has clearly demonstrated that FT-IR microspectroscopy using the mapping method can be used for diagnosis. (Chang Gung Med J 2006;29:518-27) P ancreatic cancer remains a major public health problem in the United States and other developed countries. In the U.S. in 2004, it was estimated that 31,860 new cases of pancreatic cancer would be diagnosed, with 31,270 people dying from the disease. (1) Pancreatic cancer is the fourth leading cause of cancer-related death for both men and women, and is responsible for 6% of all cancer-related deaths. (1) In Taiwan, there were 35,201 cancer deaths in 2003, accounting for 27% of all deaths. Pancreatic cancer was the tenth most prevalent cause of cancer-related deaths, being responsible for some 3% of all cancer-related deaths. (2) Patients' survival depends on the extent of the disease and the performance status on diagnosis. The extent of disease is best categorized as resectable, locally advanced, or metastatic. Owing to diagnostic difficulties, the aggressiveness of pancreatic cancers, Case Report