Internet Archive Scholar

Shotgun proteomics of SARS-CoV-2 infected cells and its application to the optimisation of whole viral particle antigen production for vaccines [article]

Lucia Grenga, Fabrice Gallais, Olivier Pible, Jean-Charles Gaillard, Duarte Gouveia, Helene Batina, Niza Bazaline, Sylvie Ruat, Karen Culotta, Guylaine Miotello, Stephanie Debroas, Marie-Anne Roncato (+8 others)
2020 bioRxiv   pre-print

Preserved Fulltext

fulltext thumbnail
Web Archive Capture PDF (1.3 MB)
https://web.archive.org/web/20200710092025/https://www.biorxiv.org/content/10.1101/2020.04.17.046193v1.full.pdf

A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2020; you can also visit the original URL. The file type is application/pdf.

Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) has resulted in a pandemic and continues to spread quickly around the globe. Currently, no effective vaccine is available to prevent COVID-19 and an intense global development activity is in progress. In this context, the different technology platforms face several challenges resulting from the involvement of a new virus still not fully characterised. Finding of the right conditions for virus amplification for the development
more » ... f vaccines based on inactivated or attenuated whole viral particles is among them. Here, we describe the establishment of a workflow based on shotgun tandem mass spectrometry data to guide the optimisation of the conditions for viral amplification. In parallel, we analysed the dynamic of the host cell proteome following SARS-CoV-2 infection providing a global overview of biological processes modulated by the virus and that could be further explored to identify drug targets to address the pandemic.
doi:10.1101/2020.04.17.046193 fatcat:v7p6a32xxzccpaj7sq7ndl7yci
Citation

Lucia Grenga, Fabrice Gallais, Olivier Pible, Jean-Charles Gaillard, Duarte Gouveia, Helene Batina, Niza Bazaline, Sylvie Ruat, Karen Culotta, Guylaine Miotello, Stephanie Debroas, Marie-Anne Roncato, Gerard Steinmetz, Charlotte Foissard, Anne Desplan, Beatrice Alpha-Bazin, Christine Almunia, Fabienne Gas, Laurent Bellanger, Jean Armengaud. "Shotgun proteomics of SARS-CoV-2 infected cells and its application to the optimisation of whole viral particle antigen production for vaccines." bioRxiv (2020)