Polymerase Chain Reaction (PCR) is widely used in biological research labs in order to detect hereditary diseases, diagnose infectious diseases, clone genes and other purposes. This work focuses on combining CFD and response surface modelling to explore the dependence of DNA amplification on two design parameters in a single phase, continuous flow PCR microfluidic device, consisting of a serpentine-like rectangular channel with three copper wire heaters. The spacing and the width of the

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... nnel between the heaters are selected as the two design parameters investigated. COMSOL Multiphysics ® 5.4 is used to simulate the performance and function of the microfluidic channel, while Design of Experiments and a polyharmonic spline are used to produce the response surface. The results indicate a ∼1.4% increase at the value of [DNA] in one PCR cycle.