Discrete Structure of an RNA Folding Intermediate Revealed by Cryo-electron Microscopy

Nathan J. Baird, Steven J. Ludtke, Htet Khant, Wah Chiu, Tao Pan, Tobin R. Sosnick
2010 Journal of the American Chemical Society  
RNA folding occurs via a series of transitions between metastable intermediate states. It is unknown whether folding intermediates are discrete structures folding along defined pathways or heterogeneous ensembles folding along broad landscapes. We use cryo-electron microscopy and single particle image reconstruction to determine the structure of the major folding intermediate of the specificity domain of a ribonuclease P ribozyme. Our results support the existence of a discrete conformation of
more » ... his folding intermediate. * trsosnic@uchicago.edu . Supporting Information Available. Cryo-EM methods and a figure describing modeling of the intermediate structure. NIH Public Access
doi:10.1021/ja107492b pmid:21038867 pmcid:PMC2988076 fatcat:v7qbst5k3bgjhlz6gw5izbnz4u