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Large-scale overproduction and rapid purification of the Escherichia coli ssb gene product. Expression of the ssb gene under .lambda. PL control
1986
Biochemistry
We report a rapid procedure for the large-scale purification of the Escherichia coli encoded single-strand binding (SSB) protein, a helix-destabilizing protein which is essential for replication, recombination, and repair processes in E. coli. To facilitate the isolation of large quantities of the ssb gene product, we have subcloned the ssb gene into a temperature-inducible expression vector, pPLc28 [Remaut, E., Stanssens, P., & Fiers, W. (1 98 1) Gene 15, 8 1-93], carrying the bacteriophage X
doi:10.1021/bi00349a004
pmid:3006753
fatcat:fwbbzm67dbgtld7tuapl3mvewu