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We report a rapid procedure for the large-scale purification of the Escherichia coli encoded single-strand binding (SSB) protein, a helix-destabilizing protein which is essential for replication, recombination, and repair processes in E. coli. To facilitate the isolation of large quantities of the ssb gene product, we have subcloned the ssb gene into a temperature-inducible expression vector, pPLc28 [Remaut, E., Stanssens, P., & Fiers, W. (1 98 1) Gene 15, 8 1-93], carrying the bacteriophage Xdoi:10.1021/bi00349a004 pmid:3006753 fatcat:fwbbzm67dbgtld7tuapl3mvewu