Immunohistochemical Localization of YAP and TAZ in Tongue Wound Healing

Noda Hajime, Tamamura Ryo, Kono Tetsuro
2019 International Journal of Oral-Medical Sciences  
Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ)are core components in development, homeostasis, and regeneration of tissues via the Hippo signaling pathway, which induces responses such as proliferation and apoptosis of cells. In recent years, the accumulation of YAP and TAZ proteins has been reported during the healing of skin wounds. However, no papers have reported YAP and TAZ expression during the healing of the oral mucosa. The present study used
more » ... resent study used immunohistochemistry(IHC)to examine the localization of YAP and TAZ during the healing of tongue ulcers in mice. The experiment animals were male ICR mice. The wound was made on each mouse's tongue and the tissue was removed upon necropsy. The wounded tissues were subjected to hematoxylin and eosin (HE) staining and to IHC staining using anti-YAP and anti-TAZ antibodies. The IHC staining was scored based on the percentage of positive cells and staining intensity; the two scores were summed to obtain a final score. Analysis targets were epithelium, fibroblasts, inflammatory cells, muscle fibers, and endothelial cells. YAP-and TAZ-positive cells were observed in the epithelium, muscle fibers, fibroblasts, inflammatory cells, and endothelial cells; high levels of YAP and TAZ expression were seen in the proliferating cells. After the ulcer formed granulated tissue and matured, YAP-and TAZ-positive cells were observed in the epithelium and fibroblasts. Those cells showed high scores during proliferation, with scores gradually decreasing as the granulated tissue matured. In conclusion, our results demonstrated that YAP and TAZ expression are associated with cell proliferation in the wound healing of the tongue.
doi:10.5466/ijoms.18.74 fatcat:djj6rmzv2jca5dcrxvsa5mpejm