G protein-coupled receptor mediated sensing of TMA

Anke Suska, Ana B. Ibáñez, Pakorn Preechaburana, Ingemar Lundström, Anna Berghard
2009 Procedia Chemistry  
A new approach for the detection of trimethylamine (TMA) using recombinant Xenopus laevis melanophores was developed. The cells were genetically modified to express the mouse trace amine-associated receptor 5 (mTAAR5), a G protein-coupled receptor from the olfactory epithelium, which conferred high sensitivity to TMA. A focused chemical screen allowed the discovery of additional, previously unknown stimuli of mTAAR5. The cell-based sensor demonstrated no sensitivity to trimethylamine Noxide
more » ... hylamine Noxide (TMAO), making it suitable for a convenient evaluation of TMA levels in fish tissue extracts. The developed gas measurement platform was able to detect TMA from 1 to 100 ppm within thirty-five minutes.
doi:10.1016/j.proche.2009.07.080 fatcat:mxgrm5juejcfpmqen6vqdtfbfu