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Breeding and preliminarily phenotyping of a congenic mouse model with alopecia areata
In the current study, the alopecia areata gene was introduced into the C57BL/6 (B6) mouse through repeated backcross-ing/intercrossing, and the allelic homozygosity of congenic AA tj mice (named B6.KM-AA) was verified using microsatellites. The gross appearance, growth characteristics, pathological changes in skin, and major organs of B6.KM-AA mice were observed. Counts and proportions of CD4 + and CD8 + T lymphocytes in peripheral blood were determined by flow cytometry. Results show thatfatcat:apogvjnaongvncjnefpprq6n5u