Evaluation of the effect of butyl p-hydroxybenzoate on the proteolytic activity and membrane function of human spermatozoa

B. L. Song, D. R. Peng, H. Y. Li, G. H. Zhang, J. Zhang, K. L. Li, Y. Q. Zhao
1991 Reproduction  
The inhibition of the proteolytic activity of acrosin in human spermatozoa by butyl p-hydroxybenzoate was assessed by the gelatin substrate film method. Compared with a typical acrosin inhibitor, TLCK, the inhibitory activity of butyl p-hydroxybenzoate to acrosin was much more effective (20 times) than that of TLCK, proving that butyl p-hydroxybenzoate was a potent acrosin inhibitor. The effect of butylp-hydroxybenzoate on membrane function of human spermatozoa was evaluated using a sperm-tail
more » ... ypoosmotic swelling test and supravital stain method. A good correlation (r = 0\m=.\92) was observed between the % spermatozoa with normal membrane function and the % live spermatozoa after treatment of the spermatozoa with butyl p-hydroxybenzoate for 1 min, indicating that the death of spermatozoa caused by butyl p-hydroxybenzoate is probably due to impairment of sperm membrane function. Both the inhibitory effect on acrosin and the adverse effect on membrane function suggest that butyl p-hydroxybenzoate could be developed as a new vaginal contraceptive.
doi:10.1530/jrf.0.0910435 pmid:2013872 fatcat:hvl2knjug5f5lgdwmhubewuonq