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Penicillamine prevents ram sperm agglutination in media that support capacitation
Ram spermatozoa are difficult to capacitate in vitro. Here we describe a further complication, the unreported phenomenon of head-to-head agglutination of ram spermatozoa following dilution in the capacitation medium Tyrodes plus albumin, lactate and pyruvate (TALP). Sperm agglutination is immediate, specific and persistent and is not associated with a loss of motility. Agglutination impedes in vitro sperm handling and analysis. So the objectives of this study were to investigate the cause ofdoi:10.1530/rep-15-0413 pmid:26705263 fatcat:5zcqsv6trngnzf6tkyyx5aorru