Intracellular redox regulation by a cystine derivative suppresses UV-induced NF-κB activation

Manabu Kitazawa, Takashi Nakano, Hiromi Chuujou, Eiji Shiojiri, Keiji Iwasaki, Kazutami Sakamoto
2002 FEBS Letters  
Nuclear factor (NF)-U UB pathways are in£uenced by the intracellular reduction^oxidation (redox) balance. While NF-U UB is activated through inhibitor (I)-U UB degradation by oxidative stress, its DNA binding is accelerated in the reduced state. We found that N,NP P-diacetyl-L-cystine dimethylester (DACDM) suppressed the UVB-induced NF-U UB binding activity at a much lower concentration (50^100 W WM) than N-acetyl-Lcysteine (NAC, 10^30 mM). While NAC suppressed the I-U UB degradation but not
more » ... DNA binding, DACDM prevented the activated NF-U UB from binding DNA, without in£uencing the I-U UB degradation. These properties of DACDM make it possible to e¡ectively regulate the intracellular redox balance. ß 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
doi:10.1016/s0014-5793(02)03152-6 pmid:12208514 fatcat:ekygukne7rg2pho5piwyzsrn34