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Comparative assessment of quantification methods for tumor tissue phosphoproteomics
With increasing sensitivity and accuracy in mass spectrometry, the tumor phosphoproteome is getting into reach. However, the selection of quantitation techniques best-suited to the biomedical question and diagnostic requirements remains a trial and error decision as no study has directly compared their performance for tumor tissue phosphoproteomics. We compared label-free quantification (LFQ), spike-in-SILAC (stable isotope labeling by amino acids in cell culture) and TMT isobaric tandem massdoi:10.1101/2022.02.07.479414 fatcat:biljto26jjaa5mlhximet2h6hu