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Multiplex Reverse Transcriptase PCR Assay for Simultaneous Detection of Three Fish Viruses †

K. Williams, S. Blake, A. Sweeney, J. T. Singer, B. L. Nicholson
1999 Journal of Clinical Microbiology  

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A multiplex reverse transcriptase (RT)-PCR assay was developed for the simultaneous detection of three different fish viruses: infectious pancreatic necrosis virus (IPNV), infectious hematopoietic necrosis virus (IHNV), and viral hemorrhagic septicemia virus (VHSV). The sensitivity levels of the multiplex RT-PCR assay were 100, 1, and 32 50% tissue culture infective doses/ml for IPNV, IHNV, and VHSV, respectively.
doi:10.1128/jcm.37.12.4139-4141.1999 fatcat:e5j2qbigznb55e4lzjcckivyum
Citation

K. Williams, S. Blake, A. Sweeney, J. T. Singer, B. L. Nicholson. "Multiplex Reverse Transcriptase PCR Assay for Simultaneous Detection of Three Fish Viruses †." Journal of Clinical Microbiology 37.12 (1999) 4139-4141