Cycle Sequencing of Filamentous Phage DNA Using a Biotinylated Primer and ΔTaq DNA Polymerase

Jianping Qiu, Huanying Zhou, Joseph F. Aceto, Thomas Kieber-Emmons
1997 BioTechniques  
We describe a rapid nonradioactive, double-stranded phage DNA sequencing method using ∆ TaqDNA polymerase in cycle reaction for phage peptide-display library screening. This procedure is specific, rapid, sensitive and safe for the sequencing of large numbers of phage peptide-display colonies. In addition, thermal cycle sequencing with this chemiluminescent image detection protocol provides an inexpensive method for completing a large amount of sequencing reactions. Consequently, this method is
more » ... specially useful for those extensively working on peptide mimics using phage display libraries.
doi:10.2144/97231st03 pmid:9232243 fatcat:xnyqdpsxqnfm7npwpo33mdirsy