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Cycle Sequencing of Filamentous Phage DNA Using a Biotinylated Primer and ΔTaq DNA Polymerase
1997
BioTechniques
Preserved Fulltext
We describe a rapid nonradioactive, double-stranded phage DNA sequencing method using ∆ TaqDNA polymerase in cycle reaction for phage peptide-display library screening. This procedure is specific, rapid, sensitive and safe for the sequencing of large numbers of phage peptide-display colonies. In addition, thermal cycle sequencing with this chemiluminescent image detection protocol provides an inexpensive method for completing a large amount of sequencing reactions. Consequently, this method is
doi:10.2144/97231st03
pmid:9232243
fatcat:xnyqdpsxqnfm7npwpo33mdirsy