Development of mutants of Gliocladium virens tolerant to benomyl

G. C. Papavizas, D. P. Roberts, K. K. Kim
1990 Canadian Journal of Microbiology (print)  
1990. Development of mutants of Gliocladium virens tolerant to benomyl. Can. J. Microbiol. 36: 484-489. Aqueous suspensions of conidia of Gliocladium virens strains GI-3 and GI-21 were exposed to both ultraviolet radiation and ethyl methanesulfonate. Two mutants of GI-3 and three of GI-21 were selected for tolerance to benomyl at 10 pg .mL-', as indicated by growth and conidial germination on benomyl-amended potato dextrose agar. The mutants differed considerably from their respective wild-type
more » ... espective wild-type strains in appearance, growth habit, sporulation, carbon-source utilization, and enzyme activity profiles. Of 10 carbon sources tested, cellobiose, xylose, and xylan were the best for growth, galactose and glucose were intermediate, and arabinose, ribose, and rhamnose were poor sources of carbon. The wild-type strains and the mutants did not utilize cellulose as the sole carbon source for growth. Two benomyl-tolerant mutants of GI-3 produced less cellulase (p-1,4-glucosidase, carboxymethylcellulase, filter-paper cellulase) than GI-3. In contrast, mutants of GI-21 produced more cellulase than the wild-type strain. Only GI-3 provided control of blight on snapbean caused by Sclerotium rolfsii. Wild-type strain GI-21 and all mutants from both strains were ineffective biocontrol agents.
doi:10.1139/m90-084 fatcat:djxuamzhvvftnffdn2bqtjtxwe