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Ligand-induced biphasic protein denaturation
1990
Journal of Biological Chemistry
The results of a thermodynamic calculation of the excess heat capacity that is based on experimental observations and that incorporates the effects of ligand binding on the two-state, thermal denaturation of a protein are presented. For a protein with a single-binding site on the native species and at subsaturating concentrations of ligand, bimodal or unimodal thermograms were computed merely by assuming a larger or smaller ligand association constant, respectively. The calculated thermograms
pmid:2318882
fatcat:t6zlhu35mvg53ovvooex4jp5w4