Role of Biofield Energy Healing Treatment Based Test Formulation on Colon Cytokines Using TNBS-Induced Ulcerative Colitis in Sprague Dawley Rats

Mahendra Kumar Trivedi
2021 Gastroenterology Medicine & Research  
A novel test formulation was formulated and the study aimed to evaluate the therapeutic effect of Biofield Energy Healing (the Trivedi Effect ® -Consciousness Energy Healing) based test formulation on the colon cytokines. Each ingredient of the test formulation was divided into two parts, one part was defined as control and the other part was described as the Biofield Energy Treated test formulation, which received Biofield Energy Healing Treatment by a renowned Biofield Energy Healer Mr.
more » ... ra Kumar Trivedi. The expression of colon C-reactive protein (CRP) and pro-inflammatory cytokines viz. tumor necrosis factor-α, (TNF-α), interleukin-6 (IL-6), interleukin-10 (IL-10), IL-23, interferon-γ (IFN-γ), and tumor growth factor-β (TGF-β) was determined by ELISA method. CRP level was significantly decreased by 38.5% (p<0.01), 22.3%, 51.2% (p<0.01), 43.9% (p<0.01), and 56.4% (p<0.01) in G5: Biofield Energy Treated test formulation; G6: Biofield treatment per se to animals (-15 days), G7 (Biofield Energy Treated test formulation at day -15), G8: Biofield Energy Treatment per se to animals plus Biofield Energy Treated test formulation (-15 day), and G9 (Biofield Treatment per se (day-15) to animals plus untreated test formulation) groups, respectively as compared with the disease control (G2) group. The TGF-β level among the tested groups such as G5, G7, G8, and G9 was significantly decreased by 19%, 28.5% (p<0.001), 19.3%, and 11.6%, respectively as compared with the G2 group. In addition, the level of IL-23 was significantly (p≤0.001) decreased by 71.1% and 62.4% in the G7 and G8 groups, respectively with respect to the G2 group. The level of IL-6 was significantly decreased by 14.9%, 45% (p<0.001), 39.3% (p<0.001), and 20.9% (p<0.001), in the G6, G7, G8, and G9 groups, respectively as compared with the G2 group. The level of TNF-α was significantly reduced by 12.2%, 30% (p<0.001), 59.1% (p<0.001), 49.2%, and 15.5% (p<0.001) in the G5, G6, G7, G8, and G9 groups, respectively as compared with the G2 group. Further, the level of IFN-γ was significantly decreased by 15.6%, 40.5% (p<0.001), 62.1% (p<0.001), 52.6% (p<0.001), and 28.4% by G5, G6, G7, G8, and G9 respectively, as compared with the G2. Besides, IL-10 level was significantly increased by 31.7% (p<0.001), 14.3%, and 18.7% (p<0.05) in the G5, G6, and G9 groups, respectively as compared with the G2. Thus, it can be concluded that the Trivedi Effect®-Consciousness Energy Healing significantly modulates the colon cytokines after treatment with the test formulation and Biofield Energy Treatment per se. Thus, the Biofield Energy Treated test formulation can be utilized against many inflammatory bowel diseases (IBDs), including Crohn's disease and ulcerative colitis, hepatic, colitis, gastrointestinal, and neurodegenerative along with various other factors such as microbial, parasitic and viral inflammatory responses.
doi:10.31031/gmr.2021.05.000616 fatcat:gctuewjddzcklftw3bbnp6cl34