Regulation of specific cell clusters in TCR-T cells responding to differential expression of tumor PD-L1 [article]

Ding Renpeng, Shang Liu, Huanyi Chen, Bin Kang, Radoje Drmanac, Ying Gu, Xuan Dong, Qianqian Gao
2020 bioRxiv   pre-print
PD-L1 signaling is important in regulating T cell function and keeping balance of tumor microenvironment, but its role in modifying TCR-T cell cytotoxicity remains unknown. MART-1-specific TCR-T cells (TCR-TMART-1) were stimulated by MEL-526 tumor cells expressing different proportions of PD-L1 and used to perform cytotoxicity assays and single-cell RNA sequencing. Percentage changes of different specific cell clusters were analyzed. The percentage of cluster HLA-DR+CD38+CD8+ was upregulated
more » ... was upregulated after antigen stimulation and tumor PD-L1 modified TCR-T cell function through downregulating the percentages of clusters HLA-DR+CD28+CD8+ and HLA-DR+CD38+CD8+ which were higher in TCR-TMART-1 than in Tnull.
doi:10.1101/2020.07.28.224816 fatcat:ooswwp35vrf33nmrsmohx3htjy