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Standardization of an Enzymometric Assay for Apolipoprotein A-I by Using Mixtures of Monoclonal Antibodies
1987
Clinical Chemistry and Laboratory Medicine
For the Standardization of human plasma apolipoprotein A-I assay two well characterized monoclonal antibody mixtures were used to develop a sandwich immunoenzymometric assay. The monoclonal antibody mixture l (A05-A17-A30) in solid phase technique was selected on the basis of its higher binding capacity of [ 125 I]HDL (41 ng per well) compared to polyclonal antibody (23 ng per well). The epitopes recognized by monoclonal antibody mixture l are surface antigenic sites of apolipoprotein A-I
doi:10.1515/cclm.1987.25.12.893
fatcat:3w6lg2ahijd7zikx6ylmvgflhy