A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2020; you can also visit the original URL.
The file type is application/pdf.
A systematic evaluation of the design, orientation, and sequence context dependencies of massively parallel reporter assays
[post]
2020
unpublished
Preserved Fulltext
Massively parallel reporter assays (MPRAs) functionally screen thousands of sequences for regulatory activity in parallel. Here, we further develop and apply a novel method to assemble and functionally test libraries of greater than 2000 of the same putative enhancers as 192-mers, 354-mers, and 678-mers. We achieved a yield of 95% for 354-mers and 84% for 678-mers. Importantly, we observe surprisingly large differences in functional activity. This work provides a framework for the experimental
doi:10.21203/rs.3.pex-1065/v1
fatcat:hme55k7ujbdt3jg4xklltldxbi