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Growth cones, the terminal structures of elongating neurites are important for development and regeneration. The second messenger cAMP regulates both processes. cAMP is also known to induce growth cone turning. By using time-lapse microscopy and release of cAMP from biological inactive caged compounds the directional response of neuronal growth cones was investigated. Intracellular gradients were induced by asymmetric release of cAMP on one side of the growth cone. Using a new method, whichdoi:10.5282/edoc.2131 fatcat:vhwdbeir5jgbdcbtw6o2bxox5m