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Genomic Islands (GIs) are commonly believed to be relics of horizontal transfer and associated with specific metabolic capacities, including virulence of the strain. Horizontal gene transfer (HGT) plays a vital role in the acquisition of GIs and the evolution and adaptation of bacterial genomes. Objective: This study designed to predict the GIs and role of HGT in evolution of Livestock-Associated Staphylococcus aureus. Methods: Genomic Islands were predicted with two methods namely, Ensembledoi:10.1042/bsr20202287 pmid:33185245 pmcid:PMC7689654 fatcat:d252mua26zdlffd3cteejchdr4