The objective of this study was to assess the knowledge, attitudes, and perceptions of Probiotic Supplements use among health sciences athlete students at King Saud University in Riyadh, Saudi Arabia. To collect data, we used a questionnaire consisting of seven closed-ended questions related to the definition, mechanism of action, demographic questions, health conditions, and diseases, and eight questions to assess the athlete students' attitudes, practices and concerns about Probiotic

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... ts. One hundred and ninetyseven athlete students from the following disciplines were interviewed: Applied Medical Sciences (n=55; 29.9%), Food and Agricultural Sciences (n=53; 26.9%) Health Sciences (n=38; 19.3%), and Preparatory Year for Medical track (n=51; 25.9%) The mean age and standard deviation (M ± SD) were 21.712 ± 2.5036. Our results showed that n=141 (71.6%) athlete students correctly identified the health benefits of Probiotic Supplements. There was a significant difference (p<0.05) between the knowledge of health sciences athlete students from the different fields (n=56; 28.4%). Other athlete students had lower knowledge scores. One hundred and sixty athlete students (81.02%) defined probiotic foods correctly, but thirty-seven (18.08%) were unable to provide an example of a probiotic food. The results of this study thus indicate the need for basic instruction in Probiotic Supplements using accessible language for this cohort of future health sciences athlete students.