Internet Archive Scholar

Preliminary Assessment of DNA Profile Patterns of Neuronal Differentiation Factor (NeuroD1) Gene in the Hippocampus of Wistar Rats (Rattus norvegicus) Ingested Hyoscyamine Fraction of Datura stramonium Seeds

Idris Abdu Tela, Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Science, Bayero University Kano, Nigeria, Sunday Abraham Musa, Ibrahim Abdullahi Iliya, Department of Human Anatomy, Faculty of Basic Medical Sciences, College of Medical Science, Ahmadu Bello University Zaria, Kaduna State, Nigeria, Department of Human Anatomy, Faculty of Basic Medical Sciences, College of Medical Science, Federal University, Dutse, Jigawa State, Nigeria
2020 Journal of Biotechnology & Bioinformatics Research  

Preserved Fulltext

fulltext thumbnail
Web Archive Capture PDF (830.9 kB)
https://web.archive.org/web/20210131142507/https://www.onlinescientificresearch.com/articles/preliminary-assessment-of-dna-profile-patterns-of-neuronal-differentiation-factor-neurod1-gene-in-the-hippocampus-of-wistar-rats-r.pdf

A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2021; you can also visit the original URL. The file type is application/pdf.

The study was designed to find out how oral ingestion of hyoscyamine fraction of D. stramonium seeds affect the DNA profiles patterns and expression of NeuroD1 gene in the hippocampus of adult Wistar rats. Fresh D. stramonium seeds were procured, identified, macerated and fractionated. Thirty-two (32) male adult Wistar rats weighed 200-250 grams were procured and divided into four groups comprised of control and three treatment groups for the study. The control received an equivalent bodyweight
more » ... of normal saline, while the treatment groups received 200, 400 and 800 mg/kgbwt of hyoscyamine fraction for three weeks respectively. The animals were sacrificed, tissues harvested for histology, gel electrophoresis and gene expression studies. The fold changes were calculated and DNA profiles were determined using LabImage bio-imaging software. One-way ANOVA followed by Fisher's multiple comparisons post-hoc test was used to obtain the difference in the expression of the gene across the groups using Minitab 17 (LLC., U.K.) statistical package software. P < 0.05 was considered statistically significant. There was a statistically significant downregulation (p = 0.001) in the NeuroD1 expression, increased DNA fragment size, decreased DNA migration distance and band volume, and necrosis of the dentate gyrus (DG) region in the hippocampus of the treated animals. In conclusion, oral ingestion of hyoscyamine fraction of D. stramonium seeds is toxic to the DNA profile patterns and expression of the NeuroD1 gene in Wistar rats.
doi:10.47363/jbbr/2020(2)122 fatcat:wr3wxe6gczgh5jlpce4cc6qhli
Citation

Idris Abdu Tela, Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Science, Bayero University Kano, Nigeria, Sunday Abraham Musa, Ibrahim Abdullahi Iliya, Department of Human Anatomy, Faculty of Basic Medical Sciences, College of Medical Science, Ahmadu Bello University Zaria, Kaduna State, Nigeria, Department of Human Anatomy, Faculty of Basic Medical Sciences, College of Medical Science, Federal University, Dutse, Jigawa State, Nigeria. "Preliminary Assessment of DNA Profile Patterns of Neuronal Differentiation Factor (NeuroD1) Gene in the Hippocampus of Wistar Rats (Rattus norvegicus) Ingested Hyoscyamine Fraction of Datura stramonium Seeds." Journal of Biotechnology & Bioinformatics Research (2020) 1-6