Obtaining purified human intestinal epithelia for single-cell analysis and organoid culture

Alexander D.B. Ross, Francesca Perrone, Rasa Elmentaite, Sarah A. Teichmann, Matthias Zilbauer
2021 STAR Protocols  
Here, we describe protocols for the preparation and dissociation of human fetal and pediatric intestinal tissue to a high-viability epithelial single-cell suspension. This epithelium-enriched single-cell suspension can then be used to generate single-cell RNA sequencing data as well as to create human intestinal organoids from both the fetal and pediatric intestine. Finally, this protocol details the dissociation of the intestinal organoids for use in single-cell analysis or passaging of
more » ... passaging of organoids. For complete details on the use and execution of this protocol, please refer to Elmentaite et al. (2020).
doi:10.1016/j.xpro.2021.100597 pmid:34169291 pmcid:PMC8209738 fatcat:4kjpewgz6nb6bmditrqfnnx7xy