Linoleic acid (LA, C18:2ω-6) and alpha linolenic acid (ALA, C18:3ω-3) were essential polyunsaturated fatty acid (PUFA). The exploration of local varieties of soybean as the sources of LA and ALA is very important to reduce the dependence of LA+ALA import products. However, the local varieties of soybean in Indonesia are limited to be ex- plored as the suorce of LA and ALA. The efficient technique is needed to increase the PUFA (LA and ALA) content of soybean oil. One of the techniques is the

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... bination of simultaneous saponification and extraction or one step saponification-extraction.This research was objected to elucidate the potency of local soybean varieties as the source of LA and ALA and to develop one step saponification-extraction in obtaining high LA+ALA oil from local varieties of soybean. Firstly, various local soybean varieties, i.e. Panderman, Wilis, Kaba, Burangrang, and Anjasmara, were assessed for fatty acid profiles. The selected variety of local soybean for one step saponification-extraction in oil extraction was based on the highest content of LA and ALA. Secondly, the condition of one step saponification-extraction was optimized by using Response Surface Methodology with three factors: water to soybean meal ratio, saponification temperature, and saponi-fication time. The response was LA+ALA content in extracted oil.The result showed that among the local soybean varieties tested, Burangrang had the highest content of LA+ALA (60.43 %). Different varieties showed different fatty acid profile and oil (in the form of fatty acid) content. Linoleic acid was the predominant in all varieties. Burangrang was used as the raw material of one step saponification-extraction. Ra- tio of water to soybean meal, saponification temperature, and time affected response of LA+ALA content. The response is quadratic. Optimum condition was achieved at water to soybean meal ratio of 2.03:1, saponification temperature of58.86 °C, and saponification time of 92.27 minutes. The response of LA+ALA concentration (%) at opti [...]