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In this study, we sought a means to increase current culture collections of SAR11 marine bacteria by testing the use of seawater cryopreserved with glycerol as an inoculum. In July 2017, raw seawater was collected outside of Kaneohe Bay, Hawai'i, in the tropical Pacific Ocean. A portion of this sample was diluted in seawater-based growth medium to create 576 x 2 mL dilution cultures containing 5 cells each and incubated for a high-throughput cultivation experiment, while another portion wasdoi:10.1101/2020.09.22.309336 fatcat:jsv4gajvzreabn7e7cxiv7u64y