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The methods of isolation, purification and qualitative and quantitative determination of phenol in air and biological fluids of an organism are offered. Isolation was carried out by distillation with water vapor, purification by extraction with chloroform, and identification by qualitative reactions, by chromatography in a thin layer of sorbent and by liquid chromatography. The quantitative determination of phenol was carried out by liquid chromatography in a reverse phase (chromatographdoi:10.32447/20786662.32.2018.11 fatcat:mdkvqj5m7nakdau2hdmkop4hki