Identification of chilli (Capsicum Annuum L.) cultivars based on chemical tests and Sds-page of total soluble proteins

Nagaraju KS, KPR Prasanna, S Rajendra Prasad, A Mohan Rao, K Vishwanath
2020 International Journal of Chemical Studies  
Identification of chilli cultivar is prime importance to ensuring seed quality during seed production, certification and quality control. Fifteen cultivars were subjected to different biochemical tests Viz., phenol test, modified phenol test with FeSO4 and CuSO4, analysis of soluble seed proteins by SDS-PAGE. No single varieties were so distinct in case of phenol colour reaction, while it helped to group the cultivars. Based on modified phenol text with ferrous sulphates 1per cent (FeSO4) and
more » ... cent (FeSO4) and 0.5 per cent copper sulphate (CuSO4) helped in further classification of cultivars. The cultivars Gouribidanur Local and Phule Jyothi showed unique bands at 14 (Rm: 0.624) and 2 (0.191) level of band. All the cultivars were distinct among each other showing SDS-PAGE as efficient method in chilli cultivar identification.
doi:10.22271/chemi.2020.v8.i5ac.10622 fatcat:l4pyc5sn6ban3jvryuju5653ma