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Seeds of two perennial ryegrass (Lolium perenne L.) cultivars (diploid 'Bartwingo' and tetraploid 'Calibra') were stored in hermetically sealed glass jars at four different temperatures (-80, -20, 10 and 20°C) for five years. After the storage period the seeds were sowed in containers filled with commercial substrate. Initial growth and development occurred under natural sunlight and manual watering to maintain optimum substrate moisture. After 60 days of vegetation plants were taken from thedoi:10.33128/s18.104.22.168 fatcat:quum22gxrzffpcnvgvy2uxe6fm