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Methods to investigate the expression of lignin peroxidase genes by the white rot fungus Phanerochaete chrysosporium
1993
Applied and Environmental Microbiology
Two methods allowing the analysis of expression of specific lignin peroxidase (LPO) genes from white rot fungi are presented. In the first method, degenerate oligonucleotide primers derived from amino acid sequence motifs held in common among all members of the LPO gene family are used to prime the polymerase chain reaction (PCR) amplification of LPO-related nucleotide sequences from cDNA prepared by using RNA from ligninolytic cultures. The PCR products are cloned and analyzed by restriction
doi:10.1128/aem.59.9.2897-2903.1993
fatcat:ao7xjxaogrggfohs66oei2aahu