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MeFiT: merging and filtering tool for illumina paired-end reads for 16S rRNA amplicon sequencing
2016
BMC Bioinformatics
Recent advances in next-generation sequencing have revolutionized genomic research. 16S rRNA amplicon sequencing using paired-end sequencing on the MiSeq platform from Illumina, Inc., is being used to characterize the composition and dynamics of extremely complex/diverse microbial communities. For this analysis on the Illumina platform, merging and quality filtering of paired-end reads are essential first steps in data analysis to ensure the accuracy and reliability of downstream analysis.
doi:10.1186/s12859-016-1358-1
pmid:27905885
pmcid:PMC5134250
fatcat:qrsocgl5jfg2zk65osmv4bujrm