Primary culture of the freshly isolated liver cells of the eel

Seiichi HAYASHI, Zentaro OOSHIRO
1985 Nippon Suisan Gakkaishi  
The primary culture of the freshly isolated liver cells of the eel was carried out. The isolated cells could attach, spread and form monolayer only on the dish which was coated with fibronectin . The cell number decreased during the culture and decreased by about 40 to 50% of the initial cell number after 7 days culture. The rate of protein synthesis increased during 8 days culture and the synthesis of the intracellular proteins was always higher than that of the extracellular proteins during
more » ... r proteins during the same period. From the investigation of synthesis of the extracellular proteins by fluorography a few proteins were synthesized and excreted by cultured hepatocytes , but the synthesis of the proteins corresponding to the bovine serum albumin was not observed. From the comparison of glucose, glycogen, and protein synthesis in the freshly isolated and cultured cells, it was found that the cultured cells had higher activity of glycogen and protein synthesis than the isolated cells. The rate of protein synthesis in the cultured cells was 10 to 100 times higher than that in the isolated cells. However, the rate of glucose synthesis in the freshly isolated liver cells was higher than that of the cultured cells.
doi:10.2331/suisan.51.765 fatcat:drknme7x2zaofofiegcxvfcs6e