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Definition of the Interaction Domain for Cytochromecon the Cytochromebc1Complex
2000
Journal of Biological Chemistry
The interaction domain for cytochrome c on the cytochrome bc 1 complex was studied using a series of Rhodobacter sphaeroides cytochrome bc 1 mutants in which acidic residues on the surface of cytochrome c 1 were substituted with neutral or basic residues. Intracomplex electron transfer was studied using a cytochrome c derivative labeled with ruthenium trisbipyridine at lysine 72 (Ru-72-Cc). Flash photolysis of a 1:1 complex between Ru-72-Cc and cytochrome bc 1 at low ionic strength resulted in
doi:10.1074/jbc.275.13.9587
pmid:10734109
fatcat:gkpj454td5cuxfjuzwsmumi72e